Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

Biobanking of Fresh-Frozen Human Adenocarcinomatous and Normal Colon Tissues: Which Parameters Influence RNA Quality?

Medical research projects become increasingly dependent on biobanked tissue of high quality because the reliability of gene expression is affected by the quality of extracted RNA. Hence, the present study aimed to determine if clinical, surgical, histological, and molecular parameters influence RNA quality of normal and tumoral frozen colonic tissues. RNA Quality Index (RQI) was evaluated on 24...

Tissue preparation for laser capture microdissection and RNA extraction from fresh frozen breast tissue.

Differences in the mechanical properties of calcaneal artificial specimens, fresh frozen specimens, and embalmed specimens in experimental testing.

BACKGROUND Artificial calcanei, fresh-frozen cadaver specimens, and embalmed cadaver specimens were compared in experimental testing under biocompatible loading to clarify the biocompatibility of artificial calcaneal specimens for implant testing. METHODS Two different artificial calcaneal bone models (Sawbone, Pacific Research Laboratories, Vashon, WA, and Synbone, Synbone Inc., Davos, Switz...

Cytoplasmic RNA extraction from fresh and frozen mammalian tissues.

The quality of collections of expressed sequence tags andfull-length cDNAs is adversely affected by the presence of "junk" clones derivedfrom unspliced or partially spliced RNAs present in conventional total RNA preparations. One can overcome this problem by using intact cytoplasmic RNA to create cDNA libraries, but the methods in the literature that describe the preparation of RNA only work we...

Gene expression analysis of normal and colorectal cancer tissue samples from fresh frozen and matched formalin-fixed, paraffin-embedded (FFPE) specimens after manual and automated RNA isolation.

Although RNA isolation is a routine process in gene expression analysis studies, the applicability of most widely available formalin-fixed, paraffin-embedded (FFPE) samples is still limited compared to fresh frozen tissue samples due to the lower quality of the isolated RNA. Recently, novel automated isolation methods were developed in order to reduce manual sample handling and increase RNA qua...